GeneTex
United States (US)

NORE1 antibody

NORE1-antibody_WB_GTX16757-1_18121411_464.jpg

Cat No. GTX16757

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

WB: Use at a dilution of 1/500 - 1/1000. Predicted molecular weight: 47 kDa. Optimal dilutions/concentrations should be determined by the end user.
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.02% Sodium Azide; Constituents: PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: DNPQKFALFKRIHKDGQ, corresponding to amino acids 313-329 of NORE1. This sequence is located in the RA domain of NORE1 and is 100% conserved between human, rat and mouse.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

RASSF1A, a new tumor-suppressor gene located at 3p21.3 is frequently inactivated by promoter region hypermethylation in a variety of human cancers including lung, breast, kidney and neuroblastoma. Another member of the RASSF1 gene family, Ras effector NORE1, which is structurally related to RASSF1, can also mediate a Ras-dependent apoptosis. Moreover, an analysis of NORE1 protein expression showed that it is frequently down-regulated in lung tumor cell lines and primary lung tumors.

Research Area

DATA IMAGES
NORE1-antibody_WB_GTX16757-1_18121411_464.jpg

GTX16757 WB Image

Western blot of anti-NORE1 performed from 50 ug of 293T cell lysates overexpressed with NORE1. Primary antibody anti-NORE1 (dilution 1/1,000) was incubated ON at 4 degree C. Second rabbit HRP antibody was incubated 1 hour at RT followed by ECL detection.