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NORE1 antibody

Anti-NORE1 antibody used in Western Blot (WB). GTX16757

Cat. No. GTX16757

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all NORE1 products

APPLICATION

Application Note

WB: Use at a dilution of 1/500 - 1/1000. Predicted molecular weight: 47 kDa. Optimal dilutions/concentrations should be determined by the end user.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: DNPQKFALFKRIHKDGQ, corresponding to amino acids 313-329 of NORE1. This sequence is located in the RA domain of NORE1 and is 100% conserved between human, rat and mouse.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

RASSF1A, a new tumor-suppressor gene located at 3p21.3 is frequently inactivated by promoter region hypermethylation in a variety of human cancers including lung, breast, kidney and neuroblastoma. Another member of the RASSF1 gene family, Ras effector NORE1, which is structurally related to RASSF1, can also mediate a Ras-dependent apoptosis. Moreover, an analysis of NORE1 protein expression showed that it is frequently down-regulated in lung tumor cell lines and primary lung tumors.

Research Area

DATA IMAGES

Anti-NORE1 antibody used in Western Blot (WB). GTX16757

GTX16757 WB Image

Western blot of anti-NORE1 performed from 50 ug of 293T cell lysates overexpressed with NORE1. Primary antibody anti-NORE1 (dilution 1/1,000) was incubated ON at 4 degree C. Second rabbit HRP antibody was incubated 1 hour at RT followed by ECL detection.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399