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NOSIP antibody [N1C3]

Anti-NOSIP antibody [N1C3] used in Western Blot (WB). GTX116126
Anti-NOSIP antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX116126
Anti-NOSIP antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116126
Anti-NOSIP antibody [N1C3] used in Western Blot (WB). GTX116126
Anti-NOSIP antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX116126

Cat. No. GTX116126

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all NOSIP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NOSIP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10621138

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nitric oxide synthase interacting protein , CGI-25

Cellular Localization

Cytoplasm , Nucleus

Background

Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity.

Database

Research Area

DATA IMAGES

Anti-NOSIP antibody [N1C3] used in Western Blot (WB). GTX116126

GTX116126 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
12% SDS PAGE
GTX116126 diluted at 1:1000

Anti-NOSIP antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX116126

GTX116126 IHC-P Image

NOSIP antibody [N1C3] detects NOSIP protein at cytoplasm and nucleus in human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
NOSIP antibody [N1C3] (GTX116126) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NOSIP antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116126

GTX116126 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using NOSIP(GTX116126) antibody at 1:200 dilution.

Anti-NOSIP antibody [N1C3] used in Western Blot (WB). GTX116126

GTX116126 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX116126 diluted at 1:2000

Anti-NOSIP antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX116126

GTX116126 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using NOSIP(GTX116126) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169