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NOSTRIN antibody

Anti-NOSTRIN antibody used in IHC (Paraffin sections) (IHC-P). GTX32144
Anti-NOSTRIN antibody used in IHC (Paraffin sections) (IHC-P). GTX32144
Anti-NOSTRIN antibody used in Western Blot (WB). GTX32144

Cat No. GTX32144

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

SW480 Cell Lysate(GTX23957)

Specificity/Sensitivity

At least four isoforms are known to exist; this antibody will detect all four isoforms.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

NOSTRIN antibody was raised against a 17 amino acid peptide near the carboxy terminus of human NOSTRIN.The immunogen is located within amino acids 430 - 480 of NOSTRIN.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nitric oxide synthase trafficking , DaIP2

Cellular Localization

Cytoplasmic vesicle,Cytoplasm, cytoskeleton

Background

Nitric oxide (NO) is a potent mediator in biologic processes such as neurotransmission, inflammatory response, and vascular homeostasis. NOSTRIN binds the enzyme responsible for NO production, endothelial NO synthase (ENOS; MIM 163729), and triggers the translocation of ENOS from the plasma membrane to vesicle-like subcellular structures, thereby attenuating ENOS-dependent NO production.[supplied by OMIM, Apr 2004]

Database

Research Area

DATA IMAGES

Anti-NOSTRIN antibody used in IHC (Paraffin sections) (IHC-P). GTX32144

GTX32144 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX32144 NOSTRIN antibody.
Working concentration : 5 μg/ml

Anti-NOSTRIN antibody used in IHC (Paraffin sections) (IHC-P). GTX32144

GTX32144 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX32144 NOSTRIN antibody.
Working concentration : 20 μg/ml

Anti-NOSTRIN antibody used in Western Blot (WB). GTX32144

GTX32144 WB Image

WB analysis of SW480 cell lysate using GTX32144 NOSTRIN antibody.
Dilution : 1μg/ml (Lane A) and 2 μg/ml (Lane B)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf