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NOTCH1 antibody [GT1099]

Anti-NOTCH1 antibody [GT1099] used in Western Blot (WB). GTX64291
Anti-NOTCH1 antibody [GT1099] used in Western Blot (WB). GTX64291
Anti-NOTCH1 antibody [GT1099] used in IHC (Paraffin sections) (IHC-P). GTX64291
Anti-NOTCH1 antibody [GT1099] used in Flow cytometry (FACS). GTX64291
Anti-NOTCH1 antibody [GT1099] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64291
Anti-NOTCH1 antibody [GT1099] used in IHC (Paraffin sections) (IHC-P). GTX64291
Anti-NOTCH1 antibody [GT1099] used in IHC (Paraffin sections) (IHC-P). GTX64291
Anti-NOTCH1 antibody [GT1099] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64291

Cat No. GTX64291

Host

Rabbit

Clonality

Monoclonal

Clone Name

GT1099

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

272 kDa. ( Note )

Observed MW

120 kDa. The observed M.W. is based on the following publication. PMID: 25990317

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

notch receptor 1 , AOS5 , AOVD1 , NOTCH1 , TAN1 , hN1 , notch 1 , Notch 1

Cellular Localization

Cell membrane,Nucleus

Background

This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-NOTCH1 antibody [GT1099] used in Western Blot (WB). GTX64291

GTX64291 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NOTCH1 antibody [GT1099] (GTX64291) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
The observed M.W. is based on the publication: PMID: 25990317

Anti-NOTCH1 antibody [GT1099] used in Western Blot (WB). GTX64291

GTX64291 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NOTCH1 antibody [GT1099] (GTX64291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-NOTCH1 antibody [GT1099] used in IHC (Paraffin sections) (IHC-P). GTX64291

GTX64291 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX64291 NOTCH1 antibody [GT1099].

Anti-NOTCH1 antibody [GT1099] used in Flow cytometry (FACS). GTX64291

GTX64291 FACS Image

FACS analysis of HeLa cells using GTX64291 NOTCH1 antibody [GT1099].
Blue : Primary antibody
Red : Isotype control

Anti-NOTCH1 antibody [GT1099] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64291

GTX64291 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX64291 NOTCH1 antibody [GT1099].
Fixation : 4% PFA
Permeabilization : 0.25% Triton X-100 in PBS
Green : Primary antibody
Blue : DAPI

Anti-NOTCH1 antibody [GT1099] used in IHC (Paraffin sections) (IHC-P). GTX64291

GTX64291 IHC-P Image

IHC-P analysis of human lung tissue using GTX64291 NOTCH1 antibody [GT1099].

Anti-NOTCH1 antibody [GT1099] used in IHC (Paraffin sections) (IHC-P). GTX64291

GTX64291 IHC-P Image

IHC-P analysis of human spleen tissue using GTX64291 NOTCH1 antibody [GT1099].

Anti-NOTCH1 antibody [GT1099] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64291

GTX64291 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX64291 NOTCH1 antibody [GT1099].
Fixation : 4% PFA
Permeabilization : 0.25% Triton X-100 in PBS
Green : Primary antibody
Blue : DAPI

REFERENCE

There are currently no references for NOTCH1 antibody [GT1099] (GTX64291). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for NOTCH1 antibody [GT1099] (GTX64291). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369