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NOTCH1 antibody [GT1224]

Recombinant antibody
Anti-NOTCH1 antibody [GT1224] used in Western Blot (WB). GTX02821
Anti-NOTCH1 antibody [GT1224] used in Immunoprecipitation (IP). GTX02821
Anti-NOTCH1 antibody [GT1224] used in Western Blot (WB). GTX02821

Cat. No. GTX02821

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1224

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse
Package
100 μl ($419)
See all NOTCH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

273 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Notch1

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

notch receptor 1 , AOS5 , AOVD1 , TAN1 , hN1

Cellular Localization

Cell membrane

Background

This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-NOTCH1 antibody [GT1224] used in Western Blot (WB). GTX02821

GTX02821 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NOTCH1 antibody [GT1224] (GTX02821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NOTCH1 antibody [GT1224] used in Immunoprecipitation (IP). GTX02821

GTX02821 IP Image

IP analysis of 293T whole cell lysate using GTX02821 NOTCH1 antibody [GT1224].
Total extract : 200μg
IP antibody amount : 3μg
Dilution : 1:1000

Anti-NOTCH1 antibody [GT1224] used in Western Blot (WB). GTX02821

GTX02821 WB Image

WB analysis of various samples using GTX02821 NOTCH1 antibody [GT1224].
Dilution : 1:1000
Loading : 25μg

REFERENCE

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REVIEW

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Package List Price ($)
$ 419