Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with NOTCH1 antibody (GTX135786) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

NOTCH1 antibody detects NOTCH1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
NOTCH1 stained by NOTCH1 antibody (GTX135786) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

NOTCH1 antibody detects NOTCH1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
NOTCH1 stained by NOTCH1 antibody (GTX135786) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NOTCH1 antibody (GTX135786) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NOTCH1 antibody detects NOTCH1 protein at cell membrane and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
NOTCH1 stained by NOTCH1 antibody (GTX135786) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min