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NOTCH1 antibody

Anti-NOTCH1 antibody used in Western Blot (WB). GTX135786
Anti-NOTCH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX135786
Anti-NOTCH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX135786
Anti-NOTCH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX135786
Anti-NOTCH1 antibody used in Western Blot (WB). GTX135786

Cat No. GTX135786

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

273 kDa. ( Note )

Product Note

The immunogen sequence of this antibody is within notch intracellular domain (NICD).

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human NOTCH1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2909883

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

notch receptor 1 , AOS5 , AOVD1 , TAN1 , hN1

Cellular Localization

Cell membrane

Background

This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-NOTCH1 antibody used in Western Blot (WB). GTX135786

GTX135786 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with NOTCH1 antibody (GTX135786) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-NOTCH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX135786

GTX135786 IHC-P Image

NOTCH1 antibody detects NOTCH1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
NOTCH1 stained by NOTCH1 antibody (GTX135786) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NOTCH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX135786

GTX135786 IHC-P Image

NOTCH1 antibody detects NOTCH1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
NOTCH1 stained by NOTCH1 antibody (GTX135786) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NOTCH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX135786

GTX135786 IHC-P Image

NOTCH1 antibody detects NOTCH1 protein at cell membrane and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
NOTCH1 stained by NOTCH1 antibody (GTX135786) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NOTCH1 antibody used in Western Blot (WB). GTX135786

GTX135786 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NOTCH1 antibody (GTX135786) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 379
$ 169