GeneTex
United States (US)

NOTCH1 antibody

GTX28387_20160330_IHC-P_18121410_446.jpg

Cat No. GTX28387

Host Rabbit
Clonality Polyclonal
Application WB, IHC-P, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000 - 1:10000
IHC-P 1:1000 - 1:5000
ELISA 1:20000 - 1:100000
Not tested in other applications.

Calculated MW

273 kDa. ( Note )

Specificity/Sensitivity

Notch1 antibody is directed against human NOTCH 1.  No reaction is detected against NOTCH 2.  No reactivity was observed against Mouse Notch.  Other species have not been tested.
PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

75mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: CQHSYSSPVDNTPSHQ, corresponding to amino acids 2488-2502 of Notch1.

Purification

Antiserum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TAN1 Antibody , NOTCH1 Antibody , HN1 Antibody

Background

Notch-1 is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.

Database

Research Area

DATA IMAGES
GTX28387_20160330_IHC-P_18121410_446.jpg

GTX28387 IHC-P Image

IHC-P analysis of human brain cerebellum tissue using Notch 1 antibody at a dilution of 1:500. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Package List Price ($)
$ 429