*Optimal dilutions/concentrations should be determined by the researcher.
|1:2000 - 1:10000
|1:1000 - 1:5000
|1:20000 - 1:100000
Not tested in other applications.
Notch1 antibody is directed against human NOTCH 1. No reaction is detected against NOTCH 2. No reactivity was observed against Mouse Notch. Other species have not been tested.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
75mg/ml(Please refer to the vial label for the specific concentration.)
Synthetic peptide: CQHSYSSPVDNTPSHQ, corresponding to amino acids 2488-2502 of Notch1.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TAN1 Antibody , NOTCH1 Antibody , HN1 Antibody
Notch-1 is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.