GeneTex
  • Country / Location Selection

United States (US)

NOTCH2 antibody [C2], C-term

Anti-NOTCH2 antibody [C2], C-term used in Western Blot (WB). GTX101593
Anti-NOTCH2 antibody [C2], C-term used in Western Blot (WB). GTX101593

Cat No. GTX101593

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ChIP-seq
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ChIP-seq Assay dependent
Not tested in other applications.

Calculated MW

265 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2

Predict Reactivity

Rat, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.09 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human NOTCH2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

notch receptor 2,AGS2,HJCYS,hN2

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Notch 2 intracellular domain: Nucleus

Background

This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-NOTCH2 antibody [C2], C-term used in Western Blot (WB). GTX101593

GTX101593 WB Image

Sample (30 µg of whole cell lysate)
A:NIH-3T3 5% SDS PAGE
GTX101593 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NOTCH2 antibody [C2], C-term used in Western Blot (WB). GTX101593

GTX101593 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NOTCH2 antibody [C2], C-term (GTX101593) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE
REVIEW
There are currently no reviews for NOTCH2 antibody [C2], C-term (GTX101593). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169