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NOTCH2 antibody [C2], C-term

Comparison
Anti-NOTCH2 antibody [C2], C-term used in Western Blot (WB). GTX101593
Anti-NOTCH2 antibody [C2], C-term used in Western Blot (WB). GTX101593

Cat. No. GTX101593

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ChIP assay, ChIP-seq

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all NOTCH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ChIP assay Assay dependent
ChIP-seq Assay dependent
Not tested in other applications.

Calculated MW

265 kDa. ( Note )

Positive Control

NIH-3T3

Predict Reactivity

Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human Notch2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241115

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

notch receptor 2 , AGS2 , HJCYS , hN2

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Notch 2 intracellular domain: Nucleus

Background

This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NOTCH2 antibody [C2], C-term used in Western Blot (WB). GTX101593

GTX101593 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with NOTCH2 antibody [C2], C-term (GTX101593) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-NOTCH2 antibody [C2], C-term used in Western Blot (WB). GTX101593

GTX101593 WB Image

Sample (30 μg of whole cell lysate)
A:NIH-3T3 5% SDS PAGE
GTX101593 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Jiao WE et al. Life Sci 2021; 284 : 119922 Notch2 suppresses the development of allergic rhinitis by promoting FOXP3 expression and Treg cell differentiation.
Application : ChIP assay
Reactivity : Mouse
Lin Z et al. Mol Ther Nucleic Acids 2018; 13 : 590-595 Epistatic Association of CD14 and NOTCH2 Genetic Polymorphisms with Biliary Atresia in a Southern Chinese Population.
Application : WB
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169