GeneTex
United States (US)

NOTCH2 antibody

GTX28927_20160330_IHC-P_18121410_510.jpg

Cat No. GTX28927

Host Rabbit
Clonality Polyclonal
Application IHC-P, ELISA
Reactivity Human, Dog, Chimpanzee
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 1:200 - 1:800
ELISA 1:30000 - 1:90000
Not tested in other applications.

Calculated MW

265 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.1% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues 2396-2409 of human Notch 2 (the total protein is 2471 aa).? A residue of cysteine was added to the amino terminal end to facilitat

Purification

Antiserum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Notch 2,Ags2,Hjcys,Hn2,Notch2

Background

Synthesized in the endoplasmic reticulum as an inactive form, which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

Database

Research Area

DATA IMAGES
GTX28927_20160330_IHC-P_18121410_510.jpg

GTX28927 IHC-P Image

IHC-P analysis of human kidney tissue using Notch 2 antibody at a dilution of 1:500. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Package List Price ($)
$ 319