GeneTex
United States (US)

NOTCH3 antibody

Anti-NOTCH3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33362
Anti-NOTCH3 antibody used in Western Blot (WB). GTX33362

Cat No. GTX33362

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:200 - 1:1000
ICC/IF 1:20 - 1:200
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

244 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A synthetic peptide of human NOTCH3

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Notch 3,Cadasil,Cadasil1,Casil,Imf2,Lmns,Notch3

Background

This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-NOTCH3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33362

GTX33362 ICC/IF Image

ICC/IF analysis of SW480 cell using GTX33362 NOTCH3 antibody.
Blue : DAPI
Dilution : 1:200

Anti-NOTCH3 antibody used in Western Blot (WB). GTX33362

GTX33362 WB Image

WB analysis of various samples using GTX33362 NOTCH3 antibody.
Dilution : 1:400
Loading : 25μg per lane

REFERENCE
Package List Price ($)
$ 329