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NOTCH4 antibody

Anti-NOTCH4 antibody used in Western Blot (WB). GTX03453
Anti-NOTCH4 antibody used in IHC (Paraffin sections) (IHC-P). GTX03453
Anti-NOTCH4 antibody used in IHC (Paraffin sections) (IHC-P). GTX03453

Cat. No. GTX03453

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all NOTCH4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

210 kDa. ( Note )

Predict Reactivity

Rabbit, Sheep, Bovine, Dog, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human NOTCH4(Accession Q99466), corresponding to amino acid residues T1488-S1508.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

notch receptor 4 , INT3

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor may play a role in vascular, renal and hepatic development. Mutations in this gene may be associated with schizophrenia. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-NOTCH4 antibody used in Western Blot (WB). GTX03453

GTX03453 WB Image

WB analysis of HepG2 whole cell lysate using GTX03453 NOTCH4 antibody. The lane on the left was treated with blocking peptide.

Anti-NOTCH4 antibody used in IHC (Paraffin sections) (IHC-P). GTX03453

GTX03453 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX03453 NOTCH4 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-NOTCH4 antibody used in IHC (Paraffin sections) (IHC-P). GTX03453

GTX03453 IHC-P Image

IHC-P analysis of rat lung tissue using GTX03453 NOTCH4 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

REFERENCE

Application Reference

Brzozowa-Zasada M et al. Int J Mol Sci 2023; 24 (8) The Clinical Application of Immunohistochemical Expression of Notch4 Protein in Patients with Colon Adenocarcinoma.
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REVIEW

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Package List Price ($)
$ 399