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NOTCH4 (cleaved Val1432) antibody

Anti-NOTCH4 (cleaved Val1432) antibody used in Western Blot (WB). GTX86910

Cat. No. GTX86910

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Monkey
Package
100 μg ($399)
See all NOTCH4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
Not tested in other applications.

Calculated MW

210 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human NOTCH4.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

RRID

AB_10721650

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

INT3,NOTCH4,notch 4,notch receptor 4

Cellular Localization

Cell membrane,Nucleus

Background

This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor may play a role in vascular, renal and hepatic development. Mutations in this gene may be associated with schizophrenia. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-NOTCH4 (cleaved Val1432) antibody used in Western Blot (WB). GTX86910

GTX86910 WB Image

WB analysis of COS-7 and 293 cells all treated with etoposide (25uM, 1hour) lysates using GTX86910 NOTCH4 (cleaved Val1432) antibody.

REFERENCE

Application Reference

Zhang S et al. Neoplasia 2014; 16 (2) : 158-67 Tumor-suppressive activity of Lunatic Fringe in prostate through differential modulation of Notch receptor activation.
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REVIEW

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Package List Price ($)
$ 399