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NOTUM antibody

Anti-NOTUM antibody used in Western Blot (WB). GTX85260
Anti-NOTUM antibody used in IHC (Paraffin sections) (IHC-P). GTX85260
Anti-NOTUM antibody used in IHC (Paraffin sections) (IHC-P). GTX85260

Cat. No. GTX85260

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all NOTUM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 1 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , Human Kidney Tissue Lysate(GTX29995)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

NOTUM antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human NOTUM.The immunogen is located within amino acids 90 - 140 of NOTUM.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

notum, palmitoleoyl-protein carboxylesterase , hNOTUM

Cellular Localization

Secreted

Background

In Drosophila, the notum gene is regulated by the Wingless pathway and encodes a secreted hydrolase that modifies heparan sulfate proteoglycans. The mammalian homolog has been shown to be able to cleave glypicans and can release GPI-anchored proteins from the mammalian cell surface. Like the Drosophilia NOTUM, the mammalian protein can act as a negative regulator of the Wnt signaling pathway. NOTUM is expressed at a low level in most mammalian tissues, although it is overexpressed in a subset of human hepatocellular carcinomas. Its transcription is regulated by b-catenin/TCF and is a target of the Wnt signaling pathway, forming a negative feedback loop that regulates the expression and activity of the Wnt pathway.

Database

Research Area

DATA IMAGES

Anti-NOTUM antibody used in Western Blot (WB). GTX85260

GTX85260 WB Image

WB analysis of human kidney tissue lysate using GTX85260 NOTUM antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

Anti-NOTUM antibody used in IHC (Paraffin sections) (IHC-P). GTX85260

GTX85260 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX85260 NOTUM antibody.
Working concentration : 20 μg/ml

Anti-NOTUM antibody used in IHC (Paraffin sections) (IHC-P). GTX85260

GTX85260 IHC-P Image

IHC-P analysis of human brain tissue using GTX85260 NOTUM antibody.
Working concentration : 2.5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399