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NOVA1 antibody

Anti-NOVA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102831
Anti-NOVA1 antibody used in Western Blot (WB). GTX102831
Anti-NOVA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102831

Cat No. GTX102831

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

PC-3

Predict Reactivity

Mouse, Rat, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human NOVA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

NOVA alternative splicing regulator 1 , Nova-1

Cellular Localization

Nucleus

Background

This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-NOVA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102831

GTX102831 ICC/IF Image

NOVA1 antibody detects NOVA1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NOVA1 stained by NOVA1 antibody (GTX102831) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NOVA1 antibody used in Western Blot (WB). GTX102831

GTX102831 WB Image

Sample (30 µg of whole cell lysate)
A: PC-3
10% SDS PAGE
GTX102831 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NOVA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102831

GTX102831 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using NOVA1(GTX102831) antibody at 1:200 dilution.

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169