GeneTex
United States (US)

NOVA1 antibody

GTX102831_39974_WB_19011506_517.jpg
GTX102831_IFA_19011506_448.jpg
GTX102831_39974_20181004_ICC_IF_R_19011519_861.jpg

Cat No. GTX102831

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

PC-3

Predict Reactivity

Mouse, Rat, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human NOVA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nova-1 antibody, NOVA1 antibody, onconeural ventral antigen 1 antibody, ventral neuron-specific protein 1 antibody, paraneoplastic Ri antigen antibody, RNA-binding protein Nova-1 antibody, neuro-oncological ventral antigen 1 antibody

Cellular Localization

Nucleus

Background

This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102831_39974_WB_19011506_517.jpg

GTX102831 WB Image

Sample (30 µg of whole cell lysate)
A: PC-3
10% SDS PAGE
GTX102831 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102831_IFA_19011506_448.jpg

GTX102831 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using NOVA1(GTX102831) antibody at 1:200 dilution.

GTX102831_39974_20181004_ICC_IF_R_19011519_861.jpg

GTX102831 ICC/IF Image

NOVA1 antibody detects NOVA1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NOVA1 stained by NOVA1 antibody (GTX102831) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169