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NOX1 antibody

Anti-NOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02577
Anti-NOX1 antibody used in Western Blot (WB). GTX02577
Anti-NOX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02577
Anti-NOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02577
Anti-NOX1 antibody used in Western Blot (WB). GTX02577
Anti-NOX1 antibody used in Western Blot (WB). GTX02577
Anti-NOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02577
Anti-NOX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02577
Anti-NOX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02577

Cat No. GTX02577

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:3000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Predict Reactivity

Zebrafish, Rabbit, Bovine, Dog, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human NOX1, corresponding to a region within C-terminal amino acids.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NADPH oxidase 1 , GP91-2 , MOX1 , NOH-1 , NOH1

Cellular Localization

Cell membrane

Background

This gene encodes a member of the NADPH oxidase family of enzymes responsible for the catalytic one-electron transfer of oxygen to generate superoxide or hydrogen peroxide. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012]

Database

Research Area

DATA IMAGES

Anti-NOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02577

GTX02577 IHC-P Image

IHC-P analysis of human kidney tissue using GTX02577 NOX1 antibody.
Antigen retireval : heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-NOX1 antibody used in Western Blot (WB). GTX02577

GTX02577 WB Image

WB analysis of mouse kidney and rat brain tissue lysates using GTX02577 NOX1 antibody.

Anti-NOX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02577

GTX02577 ICC/IF Image

ICC/IF analysis of PFA-fixed HepG2 cells using GTX02577 NOX1 antibody.
Red : Primary antibody
Blue : Nuclei
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-NOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02577

GTX02577 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX02577 NOX1 antibody.
Antigen retireval : heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-NOX1 antibody used in Western Blot (WB). GTX02577

GTX02577 WB Image

WB analysis K562 cell lysate using GTX02577 NOX1 antibody.

Anti-NOX1 antibody used in Western Blot (WB). GTX02577

GTX02577 WB Image

WB analysis of HepG2, A2780, and HeLa cell lysates using GTX02577 NOX1 antibody.

Anti-NOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02577

GTX02577 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX02577 NOX1 antibody.
Antigen retireval : heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-NOX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02577

GTX02577 ICC/IF Image

ICC/IF analysis of PFA-fixed 293 cells using GTX02577 NOX1 antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-NOX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02577

GTX02577 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX02577 NOX1 antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

REFERENCE

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REVIEW

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Package List Price ($)
$ 339