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NOX1 antibody

Anti-NOX1 antibody used in Western Blot (WB). GTX103888
Anti-NOX1 antibody used in Western Blot (WB). GTX103888
Anti-NOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103888
Anti-NOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103888
Anti-NOX1 antibody used in Western Blot (WB). GTX103888
Anti-NOX1 antibody used in Western Blot (WB). GTX103888
Anti-NOX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103888
Anti-NOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103888
Anti-NOX1 antibody used in Western Blot (WB). GTX103888

Cat. No. GTX103888

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

JC , BCL-1 , 293T , A431 , HeLa

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.14 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NOX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951012

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADPH oxidase 1 , GP91-2 , MOX1 , NOH-1 , NOH1

Cellular Localization

Membrane; Multi-pass membrane protein

Background

Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress. They are unique among ion channels with respect to their extremely high selectivity, marked temperature dependence, and unitary conductance, which is 3 orders of magnitude lower than that of most other ion channels. NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NOX1 antibody used in Western Blot (WB). GTX103888

GTX103888 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NOX1 antibody (GTX103888) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NOX1 antibody used in Western Blot (WB). GTX103888

GTX103888 WB Image

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NOX1 antibody (GTX103888) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103888

GTX103888 IHC-P Image

NOX1 antibody detects NOX1 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
NOX1 antibody (GTX103888) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103888

GTX103888 IHC-P Image

NOX1 antibody detects NOX1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
NOX1 stained by NOX1 antibody (GTX103888) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NOX1 antibody used in Western Blot (WB). GTX103888

GTX103888 WB Image

Sample (30 μg of whole cell lysate)
A: JC
B: BCL-1
7.5% SDS PAGE
GTX103888 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NOX1 antibody used in Western Blot (WB). GTX103888

GTX103888 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: A431
C: HeLa
7.5% SDS PAGE
GTX103888 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NOX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103888

GTX103888 ICC/IF Image

NOX1 antibody detects NOX1 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NOX1 protein stained by NOX1 antibody (GTX103888) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-NOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103888

GTX103888 IHC-P Image

The data was published in the journal Oxid Med Cell Longev in 2017.PMID: 29317986

Anti-NOX1 antibody used in Western Blot (WB). GTX103888

GTX103888 WB Image

The data was published in the journal Sci Rep in 2017.PMID: 29142252

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169