GeneTex
United States (US)

NOX1 antibody

GTX103888_39946_WB_M_19011506_806.jpg
GTX103888_39946_20141128_IHC_19011506_272.jpg
GTX103888_39946_IFA_19011506_539.jpg
GTX103888_39946_WB_19011506_281.jpg

Cat No. GTX103888

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

293T , A431 , HeLa , JC , BCL-1

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NOX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nadph Oxidase 1,Gp91-2,Mox1,Noh-1,Noh1,Nox1

Cellular Localization

Membrane; Multi-pass membrane protein

Background

Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress. They are unique among ion channels with respect to their extremely high selectivity, marked temperature dependence, and unitary conductance, which is 3 orders of magnitude lower than that of most other ion channels. NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103888_39946_WB_M_19011506_806.jpg

GTX103888 WB Image

Sample (30 µg of whole cell lysate)
A: JC
B: BCL-1
7.5% SDS PAGE
GTX103888 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103888_39946_20141128_IHC_19011506_272.jpg

GTX103888 IHC-P Image

NOX1 antibody detects NOX1 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
NOX1 antibody (GTX103888) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103888_39946_IFA_19011506_539.jpg

GTX103888 ICC/IF Image

NOX1 antibody detects NOX1 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NOX1 protein stained by NOX1 antibody (GTX103888) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX103888_39946_WB_19011506_281.jpg

GTX103888 WB Image

Sample (30 µg of whole cell lysate)
A: 293T
B: A431
C: HeLa
7.5% SDS PAGE
GTX103888 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169