GeneTex
United States (US)

NOX2 / gp91phox antibody, Internal

GTX88404_905_IHC-P_18122102_482.jpg

Cat No. GTX88404

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P 1-2μg/ml
IHC Assay dependent

Note :

Human Placenta shows staining of syncytiotrophoblasts.
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88404-PEP).

Predict Reactivity

Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-SYLNFARKRIKNP, from the internal region of the protein sequence according to NP_000388.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GP91PHOX Antibody , NOX2 Antibody , P91PHOX Antibody , AMCBX2 Antibody , CGD Antibody , GP911 Antibody , CYBB Antibody

Background

Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]
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Database

Research Area

DATA IMAGES
GTX88404_905_IHC-P_18122102_482.jpg

GTX88404 IHC-P Image

IHC-P analysis of human placenta using GTX88404 NOX2 / gp91phox antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml