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NOX2 / gp91phox antibody

Anti-NOX2 / gp91phox antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00785
Anti-NOX2 / gp91phox antibody used in IHC (Paraffin sections) (IHC-P). GTX00785
Anti-NOX2 / gp91phox antibody used in Western Blot (WB). GTX00785

Cat No. GTX00785

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 283-570 of human NOX2 / gp91phox (NP_000388.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

AMCBX2 , CGD , CYBB , GP911 , GP91PHOX , IMD34 , NOX2 , cytochrome b245 beta chain , p91PHOX , gp91phox , cytochrome B245 heavy chain , gp91-phox , Cytochrome B245 beta , cytochrome b-245 beta chain

Cellular Localization

Cell membrane

Background

Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-NOX2 / gp91phox antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00785

GTX00785 ICC/IF Image

ICC/IF analysis of A431 cells using GTX00785 NOX2 / gp91phox antibody.
Dilution : 1:100
Orange: Primary antibody
Blur: DAPI

Anti-NOX2 / gp91phox antibody used in IHC (Paraffin sections) (IHC-P). GTX00785

GTX00785 IHC-P Image

IHC-P analysis ofrat bone marrow using GTX00785 NOX2 / gp91phox antibody.
Dilution : 1:200

Anti-NOX2 / gp91phox antibody used in Western Blot (WB). GTX00785

GTX00785 WB Image

WB analysis of various sample lysates using GTX00785 NOX2 / gp91phox antibody.
Dilution : 1:1000
Loading : 25μg

REFERENCE

There are currently no references for NOX2 / gp91phox antibody (GTX00785). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for NOX2 / gp91phox antibody (GTX00785). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329