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NOX2 / gp91phox antibody

Comparison
Anti-NOX2 / gp91phox antibody used in IHC (Paraffin sections) (IHC-P). GTX133715
Anti-NOX2 / gp91phox antibody used in Western Blot (WB). GTX133715
Anti-NOX2 / gp91phox antibody used in Western Blot (WB). GTX133715

Cat. No. GTX133715

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NOX2 / gp91phox products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

293T , HeLa , HepG2

Predict Reactivity

Rabbit, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human NOX2 / gp91phox. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887074

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome b-245 beta chain , AMCBX2 , CGD , GP91-1 , GP91-PHOX , GP91PHOX , IMD34 , NOX2 , p91-PHOX

Cellular Localization

Membrane

Background

Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-NOX2 / gp91phox antibody used in IHC (Paraffin sections) (IHC-P). GTX133715

GTX133715 IHC-P Image

NOX2 / gp91phox antibody detects NOX2 / gp91phox protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
NOX2 / gp91phox stained by NOX2 / gp91phox antibody (GTX133715) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NOX2 / gp91phox antibody used in Western Blot (WB). GTX133715

GTX133715 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NOX2 / gp91phox antibody (GTX133715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NOX2 / gp91phox antibody used in Western Blot (WB). GTX133715

GTX133715 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with NOX2 / gp91phox antibody (GTX133715) diluted at 1:1000 and competitor's antibody (sc-5827) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

Application Reference

Mori M et al. Exp Anim 2023; 72 (2) : 233-241 Cyba and Nox2 mutant rats show different incidences of eosinophilia in the genetic background- and sex-dependent manner.
Zhao YS et al. Oxid Med Cell Longev 2021; 2021 : 8520967 Involvement of Hepcidin in Cognitive Damage Induced by Chronic Intermittent Hypoxia in Mice.
Application : WB
Reactivity : Mouse
Cheng D et al. Mol Med Rep 2020; 22 (5) : 4151-4162 Protective effects of valsartan administration on doxorubicin?induced myocardial injury in rats and the role of oxidative stress and NOX2/NOX4 signaling.
Application : WB
Reactivity : Human
Sun ZM et al. Life Sci 2020; 245 : 117362 Resveratrol protects against CIH-induced myocardial injury by targeting Nrf2 and blocking NLRP3 inflammasome activation.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169