GeneTex
United States (US)

NOX2 / gp91phox antibody

Anti-NOX2 / gp91phox antibody used in Western Blot (WB). GTX31930

Cat No. GTX31930

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

Rat Brain Tissue Lysate(GTX27942)

Specificity/Sensitivity

At least two isoforms are known to exist; this antibody will detect both isoforms. NOX2 is predicted to not cross-react with other NOX proteins.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Rabbit polyclonal NOX2 antibody was raised against a 15 amino acid peptide near the amino terminus of human NOX2.The immunogen is located within amino acids 140 - 190 of NOX2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cytochrome B-245 Beta Chain,Amcbx2,Cgd,Gp91-1,Gp91-Phox,Gp91Phox,Imd34,Nox2,P91-Phox,Cybb

Background

Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-NOX2 / gp91phox antibody used in Western Blot (WB). GTX31930

GTX31930 WB Image

WB analysis of rat brain tissue lysate using GTX31930 NOX2 / gp91phox antibody.
Working concentration : 1 μg/ml

Package List Price ($)
$ 339