NOX4 antibody [N3C3]

Cat. No. GTX121929

Reference 16

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all NOX4 products

View product citations for antibody GTX121929 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC	Assay dependent
Note : WB As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.

Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

unboiled NOX4-transfected 293T

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NOX4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10726092

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADPH oxidase 4 , KOX , KOX-1 , RENOX

Cellular Localization

Endoplasmic reticulum membrane; Multi-pass membrane protein , Cell membrane; Multi-pass membrane protein (Probable) , Cell junction , focal adhesion (Probable) , Nucleus (Probable)

Background

This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.

Database

Gene ID: 50507 NOX4
UniProt: Q9NPH5 NOX4

Research Area

DATA IMAGES

GTX121929 IHC-P Image

NOX4 antibody [N3C3] detects NOX4 protein at cytoplasm in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
NOX4 antibody [N3C3] (GTX121929) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX121929 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NOX4(GTX121929) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX121929 ICC/IF Image

NOX4 antibody [N3C3] detects NOX4 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: NOX4 protein stained by NOX4 antibody [N3C3] (GTX121929) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX121929 WB Image

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NOX4 antibody [N3C3] (GTX121929) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX121929 WB Image

SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NOX4 antibody [N3C3] (GTX121929) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.