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NOX4 antibody

Anti-NOX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX31588
Anti-NOX4 antibody used in Western Blot (WB). GTX31588
Anti-NOX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX31588

Cat. No. GTX31588

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all NOX4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

Jurkat Cell Lysate(GTX27899) , Human Spleen Tissue Slide

Product Note

At least four isoforms of NOX4 are known to exist. NOX4 is predicted to not cross-react with other NOX proteins.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

NOX4 antibody was raised against a 14 amino acid peptide near the amino terminus of human NOX4.The immunogen is located within amino acids 70 - 120 of NOX4.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADPH oxidase 4 , KOX , KOX-1 , RENOX

Cellular Localization

Cell membrane,Nucleolus

Background

This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009]

Database

Research Area

DATA IMAGES

Anti-NOX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX31588

GTX31588 IHC-P Image

IHC-P analysis of human spleen tissue using GTX31588 NOX4 antibody.
Working concentration : 20 μg/ml

Anti-NOX4 antibody used in Western Blot (WB). GTX31588

GTX31588 WB Image

WB analysis of Jurkat cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX31588 NOX4 antibody.
Working concentration : 1 μg/ml

Anti-NOX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX31588

GTX31588 IHC-P Image

IHC-P analysis of human spleen tissue using GTX31588 NOX4 antibody.
Working concentration : 5 μg/ml

REFERENCE

Application Reference

Yuan Nie et al. Gut Microbes 2021; 13 (1) : 1972746 Ursolic acid reverses liver fibrosis by inhibiting NOX4/NLRP3 inflammasome pathways and bacterial dysbiosis.
Application : WB, IHC-P
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399