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United States (US)

NP-40 (25%)

Cat No. GTX30961

Application WB, ICC/IF, ELISA, IHC
Package
50 ml ($89)
PRODUCT

Product Description

NP-40 (25%)
APPLICATION

Application Note

For diluting antibodies use 1:1000 in buffers (1 ml into 999 ml).
Washing buffer use 1:500 (1 ml into 499 ml).
The optimum dilution should be determined by the individual lab.
PROPERTIES

Form

Liquid

Buffer

This detergent is supplied as 25% in water with 0.05 % preservative.

Storage

Room temperature.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Non ionic detergents like NP40 are used in immunoassays for diluting antibodies to lower background, and non-specific antigen-antibody (ag-ab) reaction. This detergent is also used in washing buffer from 0.1- 0.5% in all immunoassays (ELISA, Immunohistochemistry (IHC), Blot assays and others) to reduce background. Tergitol-type NP-40, which is nonyl phenoxylpolyethoxylethanol, lower surface tension of water.
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Package List Price ($)
$ 89