PRODUCT
APPLICATION
Application Note
For diluting antibodies use 1:1000 in buffers (1 ml into 999 ml).
Washing buffer use 1:500 (1 ml into 499 ml).
The optimum dilution should be determined by the individual lab.
PROPERTIES
Form
Liquid
Buffer
This detergent is supplied as 25% in water.
Preservative
0.05% preservative, not contain azide or mercury preservative
Storage
Store at room temperature.
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Background
Non ionic detergents like NP40 are used in immunoassays for diluting antibodies to lower background, and non-specific antigen-antibody (ag-ab) reaction. This detergent is also used in washing buffer from 0.1- 0.5% in all immunoassays (ELISA, Immunohistochemistry (IHC), Blot assays and others) to reduce background. Tergitol-type NP-40, which is nonyl phenoxylpolyethoxylethanol, lower surface tension of water.
REFERENCE
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REVIEW
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