GeneTex
United States (US)

NPAS1 antibody

GTX105876_41101_IHC_M_2_19011506_721.jpg
GTX105876_41101_IFA_19011506_153.jpg
GTX105876_41101_IHC_R_19011506_356.jpg
GTX105876_41101_WB_19011506_610.jpg
GTX105876_41101_IHC_M_19011506_945.jpg

Cat No. GTX105876

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

U87-MG , IMR32 , SK-N-AS

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NPAS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

bHLHe11 Antibody, MOP5 Antibody, PASD5 Antibody, neuronal PAS domain protein 1 Antibody

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH)-PAS family of transcription factors. Studies of a related mouse gene suggest that it functions in neurons. The exact function of this gene is unclear, but it may play protective or modulatory roles during late embryogenesis and postnatal development. [provided by RefSeq]

Database

DATA IMAGES
GTX105876_41101_IHC_M_2_19011506_721.jpg

GTX105876 IHC-P Image

NPAS1 antibody detects NPAS1 protein at nucleus on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
NPAS1 antibody (GTX105876) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105876_41101_IFA_19011506_153.jpg

GTX105876 ICC/IF Image

NPAS1 antibody detects NPAS1 protein at nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: NPAS1 protein stained by NPAS1 antibody (GTX105876) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX105876_41101_IHC_R_19011506_356.jpg

GTX105876 IHC-P Image

NPAS1 antibody detects NPAS1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
NPAS1 antibody (GTX105876) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105876_41101_WB_19011506_610.jpg

GTX105876 WB Image

NPAS1 antibody detects NPAS1 protein by Western blot analysis.
A. 30 µg U87-MG whole cell lysate/extract
B. 30 µg IMR32 whole cell lysate/extract
C. 30 µg SK-N-AS whole cell lysate/extract
7.5 % SDS-PAGE
NPAS1 antibody (GTX105876) dilution: 1:1000

GTX105876_41101_IHC_M_19011506_945.jpg

GTX105876 IHC-P Image

NPAS1 antibody detects NPAS1 protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
NPAS1 antibody (GTX105876) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169