GeneTex
  • Country / Location Selection

United States (US)

NPAS2 antibody [C1C3]

Anti-NPAS2 antibody [C1C3] used in IHC (Frozen sections) (IHC-Fr). GTX105741
Anti-NPAS2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105741
Anti-NPAS2 antibody [C1C3] used in Western Blot (WB). GTX105741
Anti-NPAS2 antibody [C1C3] used in Western Blot (WB). GTX105741
Anti-NPAS2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105741
Anti-NPAS2 antibody [C1C3] used in Western Blot (WB). GTX105741
Anti-NPAS2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105741

Cat No. GTX105741

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human NPAS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

neuronal PAS domain protein 2 , MOP4 , PASD4 , bHLHe9

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH)-PAS family of transcription factors. A similar mouse protein may play a regulatory role in the acquisition of specific types of memory. It also may function as a part of a molecular clock operative in the mammalian forebrain. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-NPAS2 antibody [C1C3] used in IHC (Frozen sections) (IHC-Fr). GTX105741

GTX105741 IHC-Fr Image

NPAS2 antibody [C1C3] detects NPAS2 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: NPAS2 protein stained by NPAS2 antibody [C1C3] (GTX105741) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NPAS2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105741

GTX105741 IHC-P Image

NPAS2 antibody [C1C3] detects NPAS2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: NPAS2 stained by NPAS2 antibody [C1C3] (GTX105741) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NPAS2 antibody [C1C3] used in Western Blot (WB). GTX105741

GTX105741 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NPAS2 antibody [C1C3] (GTX105741) diluted at 1:1000.

Anti-NPAS2 antibody [C1C3] used in Western Blot (WB). GTX105741

GTX105741 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NPAS2 antibody [C1C3] (GTX105741) diluted at 1:1000.

Anti-NPAS2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105741

GTX105741 ICC/IF Image

NPAS2 antibody [C1C3] detects NPAS2 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NPAS2 protein stained by NPAS2 antibody [C1C3] (GTX105741) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.

Anti-NPAS2 antibody [C1C3] used in Western Blot (WB). GTX105741

GTX105741 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
C: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX105741 diluted at 1:1000

Anti-NPAS2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105741

GTX105741 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using NPAS2(GTX105741) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
There are currently no references for NPAS2 antibody [C1C3] (GTX105741). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for NPAS2 antibody [C1C3] (GTX105741). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169
Currently in production