APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5 - 2 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Brain Tissue Slide(GTX22203) , Human Brain Tissue Lysate(GTX27918) , Rat Brain Tissue Lysate(GTX27942)
Product Note
This antibody is specific for NPAS3 Internal
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
NPAS3 antibody was raised against a 28 amino acid synthetic peptide from near the center of human NPAS3.The immunogen is located within amino acids 470 - 520 of NPAS3.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
neuronal PAS domain protein 3 , MOP6 , PASD6 , bHLHe12
Cellular Localization
Nucleus
Background
Neuronal PAS domain protein 3 (NPAS3) is a brain-enriched basic helix-loop-helix PAS domain transcription factor and is broadly expressed in the developing neuroepithelium and has recently found to be disrupted by genetic translocation in a family affected with schizophrenia. It was recently shown to be involved in the regulation of FGF signaling in the dentate gyrus by controlling the expression of the FGF receptor subtype 1 and in turn neurogenesis emanating from this region. NPAS3- mice were growth-retarded and displayed brain defects that included reduced size of the anterior hippocampus, hypoplasia of the corpus callosum, and enlargement of the ventricles, as well as several behavioral abnormalities. Furthermore, these NPAS3- mice also exhibited disruptions in several neurosignaling pathways involving glutamate, dopamine, and serotonin. These results demonstrate the essential role played by NPAS3 during structural and functional brain development. At least three isoforms of NPAS3 are known to exist.
Database
Research Area
DATA IMAGES
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GTX85444 IHC-P Image
IHC-P analysis of human brain tissue using GTX85444 NPAS3 antibody.
Working concentration : 20 μg/ml
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GTX85444 IHC-P Image
IHC-P analysis of human brain tissue using GTX85444 NPAS3 antibody.
Working concentration : 5 μg/ml
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GTX85444 WB Image
WB analysis of rat brain tissue lysate using GTX85444 NPAS3 antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml
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REFERENCE
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REVIEW
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