GeneTex

  • Country / Location Selection

United States (US)

NPC1 antibody

Anti-NPC1 antibody used in Western Blot (WB). GTX30687
Anti-NPC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX30687
Anti-NPC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30687
Anti-NPC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30687

Cat. No. GTX30687

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, EM

Reactivity

 Human, Mouse, Rat, Hamster, Pig, Primate
Package
100 μl ($419)
See all NPC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:3000
ICC/IF 1:250
IHC-P 5 - 10 μg/ml
EM Assay dependent
Not tested in other applications.

Calculated MW

142 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide made to the C-terminal region of human Niemann-Pick C. [UniProt# O15118]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NPC intracellular cholesterol transporter 1 , NPC , POGZ , SLC65A1

Cellular Localization

Lysosome membrane

Background

This gene was identified as the gene that when mutated, results in Niemann-Pick C disease. The encoded protein is a putative integral membrane protein that contains motifs consistent with a role in intracellular transport of cholesterol to post-lysosomal destinations. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NPC1 antibody used in Western Blot (WB). GTX30687

GTX30687 WB Image

WB analysis of human fibroblast cell lysate using GTX30687 NPC1 antibody.
Loading : 20μg

Anti-NPC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX30687

GTX30687 IHC-P Image

IHC-P analysis of human brain tissue (cortex, neurons and astrocyte) using GTX30687 NPC1 antibody.

Anti-NPC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30687

GTX30687 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30687 NPC1 antibody.
Green : primary antibody
Blue : DAPI

Anti-NPC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30687

GTX30687 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30687 NPC1 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 5 μg/ml

REFERENCE

Clone Reference

Frolov A et al. J Biol Chem 2001; 276 (49):46414-21 Cholesterol overload promotes morphogenesis of a Niemann-Pick C (NPC)-like compartment independent of inhibition of NPC1 or HE1/NPC2 function.
Millard EE et al. J Biol Chem 2000; 275 (49):38445-51 Niemann-pick type C1 (NPC1) overexpression alters cellular cholesterol homeostasis.
Submit a Reference

REVIEW

There are currently no reviews for NPC1 antibody (GTX30687). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419