GeneTex
United States (US)

NPC1 antibody

GTX30687_243_ICC-IF_18121410_950.jpg
GTX30687_244_ICC-IF_18121410_764.jpg
GTX30687_1485_WB_18121410_656.jpg
GTX30687_571_IHC-P_18121410_426.jpg

Cat No. GTX30687

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, EM
Reactivity Human, Mouse, Rat, Hamster, Pig, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000 - 1:3000
ICC/IF 1:250
IHC-P 5 - 10 μg/ml
EM Assay dependent
Not tested in other applications.

Calculated MW

142 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide made to the C-terminal region of human Niemann-Pick C. [UniProt# O15118]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

NPC antibody

Cellular Localization

Late endosome membrane, Multi-pass membrane protein,Lysosome membrane, Multi-pass membrane protein

Background

This gene was identified as the gene that when mutated, results in Niemann-Pick C disease. The encoded protein is a putative integral membrane protein that contains motifs consistent with a role in intracellular transport of cholesterol to post-lysosomal destinations. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30687_243_ICC-IF_18121410_950.jpg

GTX30687 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30687 NPC1 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 5 μg/ml

GTX30687_244_ICC-IF_18121410_764.jpg

GTX30687 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30687 NPC1 antibody.
Green : primary antibody
Blue : DAPI

GTX30687_1485_WB_18121410_656.jpg

GTX30687 WB Image

WB analysis of human fibroblast cell lysate using GTX30687 NPC1 antibody.
Loading : 20μg

GTX30687_571_IHC-P_18121410_426.jpg

GTX30687 IHC-P Image

IHC-P analysis of human brain tissue (cortex, neurons and astrocyte) using GTX30687 NPC1 antibody.

REFERENCE