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NPC1 antibody

Anti-NPC1 antibody used in Western Blot (WB). GTX31790
Anti-NPC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31790
Anti-NPC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31790

Cat. No. GTX31790

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all NPC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

142 kDa. ( Note )

Positive Control

HepG2 Cell Lysate(GTX27900)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

NPC1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human NPC1.The immunogen is located within the last 50 amino acids of NPC1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NPC intracellular cholesterol transporter 1 , NPC , POGZ , SLC65A1

Cellular Localization

Lysosome membrane

Background

This gene encodes a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. It is predicted to have a cytoplasmic C-terminus, 13 transmembrane domains, and 3 large loops in the lumen of the endosome - the last loop being at the N-terminus. This protein transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolized and released as free cholesterol. Defects in this gene cause Niemann-Pick type C disease, a rare autosomal recessive neurodegenerative disorder characterized by over accumulation of cholesterol and glycosphingolipids in late endosomal/lysosomal compartments.[provided by RefSeq, Aug 2009]

Database

Research Area

DATA IMAGES

Anti-NPC1 antibody used in Western Blot (WB). GTX31790

GTX31790 WB Image

WB analysis of HepG2 cell lysate using GTX31790 NPC1 antibody.
Working concentration : 1 μg/ml

Anti-NPC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31790

GTX31790 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX31790 NPC1 antibody.
Working concentration : 2.5 μg/ml

Anti-NPC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31790

GTX31790 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX31790 NPC1 antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399