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NPC2 antibody [N1C3]

Anti-NPC2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109217
Anti-NPC2 antibody [N1C3] used in Western Blot (WB). GTX109217

Cat. No. GTX109217

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NPC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human NPC2 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167284

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NPC intracellular cholesterol transporter 2 , EDDM1 , HE1

Cellular Localization

Secreted

Background

This gene encodes a protein containing a lipid recognition domain. The encoded protein may function in regulating the transport of cholesterol through the late endosomal/lysosomal system. Mutations in this gene have been associated with Niemann-Pick disease, type C2 and frontal lobe atrophy. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NPC2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109217

GTX109217 IHC-P Image

NPC2 antibody [N1C3] detects NPC2 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded lung carcinoma.
NPC2 antibody [N1C3] (GTX109217) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NPC2 antibody [N1C3] used in Western Blot (WB). GTX109217

GTX109217 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
15% SDS PAGE
GTX109217 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169