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NPEPPS antibody [HL1531]

Recombinant antibody
Anti-NPEPPS antibody [HL1531] used in Western Blot (WB). GTX637005
Anti-NPEPPS antibody [HL1531] used in IHC (Paraffin sections) (IHC-P). GTX637005
Anti-NPEPPS antibody [HL1531] used in Western Blot (WB). GTX637005
Anti-NPEPPS antibody [HL1531] used in Western Blot (WB). GTX637005
Anti-NPEPPS antibody [HL1531] used in Western Blot (WB). GTX637005
Anti-NPEPPS antibody [HL1531] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637005
Anti-NPEPPS antibody [HL1531] used in IHC (Paraffin sections) (IHC-P). GTX637005

Cat. No. GTX637005

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1531

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all NPEPPS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NPEPPS. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aminopeptidase puromycin sensitive , AAP-S , MP100 , PSA

Cellular Localization

Cytoplasm, cytosol,Nucleus

Background

This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-NPEPPS antibody [HL1531] used in Western Blot (WB). GTX637005

GTX637005 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS antibody [HL1531] (GTX637005) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NPEPPS antibody [HL1531] used in IHC (Paraffin sections) (IHC-P). GTX637005

GTX637005 IHC-P Image

NPEPPS antibody [HL1531] detects NPEPPS protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
NPEPPS stained by NPEPPS antibody [HL1531] (GTX637005) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NPEPPS antibody [HL1531] used in Western Blot (WB). GTX637005

GTX637005 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS antibody [HL1531] (GTX637005) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NPEPPS antibody [HL1531] used in Western Blot (WB). GTX637005

GTX637005 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS antibody [HL1531] (GTX637005) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NPEPPS antibody [HL1531] used in Western Blot (WB). GTX637005

GTX637005 WB Image

Whole zebrafish extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS antibody [HL1531] (GTX637005) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NPEPPS antibody [HL1531] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637005

GTX637005 ICC/IF Image

NPEPPS antibody [HL1531] detects NPEPPS protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: NPEPPS stained by NPEPPS antibody [HL1531] (GTX637005) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
The observed subcellular location is based on the publication: PMID: 28259985

Anti-NPEPPS antibody [HL1531] used in IHC (Paraffin sections) (IHC-P). GTX637005

GTX637005 IHC-P Image

NPEPPS antibody [HL1531] detects NPEPPS protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
NPEPPS stained by NPEPPS antibody [HL1531] (GTX637005) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations