GeneTex
United States (US)

NPEPPS antibody [N3C2-2], Internal

GTX115247_WB_18121410_444.jpg
GTX115247_40254_IFA_18121410_224.jpg
GTX115247_40254_WB_M_brain_18121410_730.jpg

Cat No. GTX115247

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116 , mouse brain

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NPEPPS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Aminopeptidase Puromycin Sensitive,Aap-S,Mp100,Psa,Npepps

Cellular Localization

Cytoplasm (Potential) , Nucleus

Background

This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. [provided by RefSeq]

Database

DATA IMAGES
GTX115247_WB_18121410_444.jpg

GTX115247 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa
7.5% SDS PAGE
GTX115247 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115247_40254_IFA_18121410_224.jpg

GTX115247 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NPEPPS(GTX115247) antibody at 1:200 dilution.

GTX115247_40254_WB_M_brain_18121410_730.jpg

GTX115247 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX115247 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169