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NPEPPS antibody [N3C2-2], Internal

Anti-NPEPPS antibody [N3C2-2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115247
Anti-NPEPPS antibody [N3C2-2], Internal used in Western Blot (WB). GTX115247
Anti-NPEPPS antibody [N3C2-2], Internal used in Western Blot (WB). GTX115247

Cat. No. GTX115247

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NPEPPS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Predict Reactivity

Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NPEPPS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619289

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aminopeptidase puromycin sensitive , AAP-S , MP100 , PSA

Cellular Localization

Cytoplasm (Potential) , Nucleus

Background

This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NPEPPS antibody [N3C2-2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115247

GTX115247 ICC/IF Image

NPEPPS antibody [N3C2-2], Internal detects NPEPPS protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NPEPPS stained by NPEPPS antibody [N3C2-2], Internal (GTX115247) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-NPEPPS antibody [N3C2-2], Internal used in Western Blot (WB). GTX115247

GTX115247 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS antibody [N3C2-2], Internal (GTX115247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NPEPPS antibody [N3C2-2], Internal used in Western Blot (WB). GTX115247

GTX115247 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS antibody [N3C2-2], Internal (GTX115247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169