GeneTex
United States (US)

NPHP1 antibody

Cat No. GTX11501

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 2-10 μg/ml
IP 10-20 μg/ml
Not tested in other applications.

Calculated MW

77 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Mouse

Immunogen

GST fusion protein corresponding to amino acids 612-691 of mouse NPHP1.

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nephronophthisis 1 (Juvenile) Homolog (Human),Nphp1

Background

Juvenile nephronophthsis, an autosomal recessive cystic kidney disease, is the primary cause for chronic renal failure in children. The gene for nephronophthsis type 1 (NPHP1) has recently been identified. Its gene product, nephrocystin, is a novel protein of unknown function, which contains a src-homology 3 domain.NPHP1 expression has been studied in mouse and human systems. Expression in the human adult is widespread but relatively weak. In contrast, expression in adult mouse is strong and localized to the testis. In situ hybridization to whole mouse embryo demonstrates widespread and consistently strong expression throughout all developmental stages.It is hypothesized that the NPHP1 gene product, nephrocystin, is involved in signaling processes at focal adhesions (cell and extracellular matrix contact) and/or adherens junctions (cell-cell contact). The hypothesis is based on the fact that most src-homology 3 -containing proteins are part of focal adhesion signaling complexes. Additionally, nephrocystin binds to p130(cas), a prominent participant in focal adhesion signaling.

Database

Research Area

Package List Price ($)
$ 289