Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NPR-A antibody [C1C3-2] (GTX110112) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Sample (30 μg of whole cell lysate)
A: HeLa
7.5% SDS PAGE
GTX110112 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.