APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human NQO1 expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
NAD(P)H quinone dehydrogenase 1 , DHQU , DIA4 , DTD , NMOR1 , NMORI , QR1
Cellular Localization
Cytoplasm
Background
This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-NQO1 antibody [4D12] used in ELISA (ELISA). GTX60523](/templates/default/js/lightbox/images/loading.gif "Anti-NQO1 antibody [4D12] used in ELISA (ELISA). GTX60523")
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GTX60523 ELISA Image
ELISA analysis of antigen using GTX60523 NQO1 antibody [4D12].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
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GTX60523 IHC-P Image
IHC-P analysis of human testis tissue using GTX60523 NQO1 antibody [4D12].
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GTX60523 IHC-P Image
IHC-P analysis of ovarian cancer tissue using GTX60523 NQO1 antibody [4D12].
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GTX60523 WB Image
WB analysis of A549 (1), SKNES (2), HepG2 (3), MCF-7 (4) and HeLa (5) cell lysate using GTX60523 NQO1 antibody [4D12].
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GTX60523 WB Image
WB analysis of human NQO1 (AA: 134-274) recombinant protein using GTX60523 NQO1 antibody [4D12].
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GTX60523 FACS Image
FACS analysis of NIH3T3 cells using GTX60523 NQO1 antibody [4D12].
Green : NQO1
Red : negative control
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REFERENCE
There are currently no references for NQO1 antibody [4D12] (GTX60523). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for NQO1 antibody [4D12] (GTX60523). Be the first to share your experience with this product.
![Anti-NQO1 antibody [4D12] used in ELISA (ELISA). GTX60523](/upload/website/prouct_img/small/GTX60523/GTX60523_20170912_ELISA_w_23061123_645.webp "Anti-NQO1 antibody [4D12] used in ELISA (ELISA). GTX60523")
![Anti-NQO1 antibody [4D12] used in IHC (Paraffin sections) (IHC-P). GTX60523](/upload/website/prouct_img/small/GTX60523/GTX60523_20170912_IHC-P_1_w_23061123_738.webp "Anti-NQO1 antibody [4D12] used in IHC (Paraffin sections) (IHC-P). GTX60523")
![Anti-NQO1 antibody [4D12] used in IHC (Paraffin sections) (IHC-P). GTX60523](/upload/website/prouct_img/small/GTX60523/GTX60523_20170912_IHC-P_w_23061123_846.webp "Anti-NQO1 antibody [4D12] used in IHC (Paraffin sections) (IHC-P). GTX60523")
![Anti-NQO1 antibody [4D12] used in Western Blot (WB). GTX60523](/upload/website/prouct_img/small/GTX60523/GTX60523_20170912_WB_w_23061123_748.webp "Anti-NQO1 antibody [4D12] used in Western Blot (WB). GTX60523")
![Anti-NQO1 antibody [4D12] used in Western Blot (WB). GTX60523](/upload/website/prouct_img/small/GTX60523/GTX60523_20170912_WB_1_w_23061123_186.webp "Anti-NQO1 antibody [4D12] used in Western Blot (WB). GTX60523")
![Anti-NQO1 antibody [4D12] used in Flow cytometry (FACS). GTX60523](/upload/website/prouct_img/small/GTX60523/GTX60523_20170912_FACS_w_23061123_474.webp "Anti-NQO1 antibody [4D12] used in Flow cytometry (FACS). GTX60523")
![Anti-NQO1 antibody [4D12] used in ELISA (ELISA). GTX60523](/upload/website/prouct_img/normal/GTX60523/GTX60523_20170912_ELISA_w_23061123_645.webp "Anti-NQO1 antibody [4D12] used in ELISA (ELISA). GTX60523")
![Anti-NQO1 antibody [4D12] used in IHC (Paraffin sections) (IHC-P). GTX60523](/upload/website/prouct_img/normal/GTX60523/GTX60523_20170912_IHC-P_1_w_23061123_738.webp "Anti-NQO1 antibody [4D12] used in IHC (Paraffin sections) (IHC-P). GTX60523")
![Anti-NQO1 antibody [4D12] used in IHC (Paraffin sections) (IHC-P). GTX60523](/upload/website/prouct_img/normal/GTX60523/GTX60523_20170912_IHC-P_w_23061123_846.webp "Anti-NQO1 antibody [4D12] used in IHC (Paraffin sections) (IHC-P). GTX60523")
![Anti-NQO1 antibody [4D12] used in Western Blot (WB). GTX60523](/upload/website/prouct_img/normal/GTX60523/GTX60523_20170912_WB_w_23061123_748.webp "Anti-NQO1 antibody [4D12] used in Western Blot (WB). GTX60523")
![Anti-NQO1 antibody [4D12] used in Western Blot (WB). GTX60523](/upload/website/prouct_img/normal/GTX60523/GTX60523_20170912_WB_1_w_23061123_186.webp "Anti-NQO1 antibody [4D12] used in Western Blot (WB). GTX60523")
![Anti-NQO1 antibody [4D12] used in Flow cytometry (FACS). GTX60523](/upload/website/prouct_img/normal/GTX60523/GTX60523_20170912_FACS_w_23061123_474.webp "Anti-NQO1 antibody [4D12] used in Flow cytometry (FACS). GTX60523")