GeneTex
United States (US)

NQO1 antibody [A180]

GTX30626_562_IHC-P_18121410_984.jpg
GTX30626_1436_WB_18121410_357.jpg
GTX30626_217_ICC-IF_18121410_540.jpg
GTX30626_102_FACS_18121410_854.jpg
GTX30626_1437_WB_18121410_899.jpg
GTX30626_1620_IHC-P_18121410_857.jpg
GTX30626_103_FACS_18121410_881.jpg

Cat No. GTX30626

Host Mouse
Clonality Monoclonal
Clone Name A180
Isotype IgG1
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP
Reactivity Human, Mouse, Rat, Dog, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:50
IHC-P 1:100
IHC-Fr 1:100
FACS 1 - 2 μg/mL
IP 1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Specificity/Sensitivity

Does not cross-react with NQO2.
PROPERTIES

Form

Liquid

Buffer

Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length recombinant NQO1 from human lung [UniProt# P15559]

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DHQU antibody , DIA4 antibody , DTD antibody , NMOR1 antibody , NMORI antibody , QR1 antibody

Cellular Localization

Cytoplasm

Background

This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30626_562_IHC-P_18121410_984.jpg

GTX30626 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX30626 NQO1 antibody [A180].

GTX30626_1436_WB_18121410_357.jpg

GTX30626 WB Image

WB analysis of A431 cell lysate using GTX30626 NQO1 antibody [A180].

GTX30626_217_ICC-IF_18121410_540.jpg

GTX30626 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX30626 NQO1 antibody [A180].
Green : primary antibody
Red : Beta-tubulin
Blue : DAPI

GTX30626_102_FACS_18121410_854.jpg

GTX30626 FACS Image

FACS (Intracellular staining) analysis of A549 cells using GTX30626 NQO1 antibody [A180].
Filled histogram : primary antibody
Open histogram : isotype control

GTX30626_1437_WB_18121410_899.jpg

GTX30626 WB Image

WB analysis of A549 cell lysate using GTX30626 NQO1 antibody [A180].
Lane 1 : 0.5 ug/ml
Lane 2 : 2 ug/ml
Dilution : 2 μg/ml

GTX30626_1620_IHC-P_18121410_857.jpg

GTX30626 IHC-P Image

IHC-P analysis of human kidney tissue using GTX30626 NQO1 antibody [A180].

GTX30626_103_FACS_18121410_881.jpg

GTX30626 FACS Image

FACS (Intracellular staining) analysis of U87-MG cells using GTX30626 NQO1 antibody [A180].
Blue : Primary antibody
Orange : isotype control
Dilution : 1 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin

Package List Price ($)
$ 329