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NQO1 antibody [GT1171]

Recombinant antibody
Anti-NQO1 antibody [GT1171] used in Western Blot (WB). GTX09504
Anti-NQO1 antibody [GT1171] used in Immunoprecipitation (IP). GTX09504
Anti-NQO1 antibody [GT1171] used in IHC (Paraffin sections) (IHC-P). GTX09504
Anti-NQO1 antibody [GT1171] used in Western Blot (WB). GTX09504

Cat. No. GTX09504

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1171

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all NQO1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from hmuan NQO1

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NAD(P)H quinone dehydrogenase 1 , DHQU , DIA4 , DTD , NMOR1 , NMORI , QR1

Cellular Localization

Cytoplasm

Background

This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-NQO1 antibody [GT1171] used in Western Blot (WB). GTX09504

GTX09504 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NQO1 antibody [GT1171] (GTX09504) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NQO1 antibody [GT1171] used in Immunoprecipitation (IP). GTX09504

GTX09504 IP Image

IP analysis of HeLa cell lysate using GTX09504 NQO1 antibody [GT1171].
Antibody amount : 3μg / 300μg lysate
Dilution : 1:1000

Anti-NQO1 antibody [GT1171] used in IHC (Paraffin sections) (IHC-P). GTX09504

GTX09504 IHC-P Image

IHC-P analysis of mouse kidney tissue section using GTX09504 NQO1 antibody [GT1171].
Dlution : 1:100

Anti-NQO1 antibody [GT1171] used in Western Blot (WB). GTX09504

GTX09504 WB Image

WB analysis of various samples using GTX09504 NQO1 antibody [GT1171].
Dilution : 1:1000
Loading : 25 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399