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NQO1 antibody

Anti-NQO1 antibody used in Western Blot (WB). GTX113336
Anti-NQO1 antibody used in Western Blot (WB). GTX113336
Anti-NQO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113336

Cat. No. GTX113336

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all NQO1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

mouse heart , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Guinea pig, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NQO1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037546

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NAD(P)H quinone dehydrogenase 1 , DHQU , DIA4 , DTD , NMOR1 , NMORI , QR1

Cellular Localization

Cytoplasm

Background

This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NQO1 antibody used in Western Blot (WB). GTX113336

GTX113336 WB Image

NQO1 antibody detects NQO1 protein by Western blot analysis.
A. 50 μg mouse heart lysate/extract
12 % SDS-PAGE
NQO1 antibody (GTX113336) dilution: 1:500

Anti-NQO1 antibody used in Western Blot (WB). GTX113336

GTX113336 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
12% SDS PAGE
GTX113336 diluted at 1:1000

Anti-NQO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113336

GTX113336 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using NQO1(GTX113336) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169