GeneTex
United States (US)

NQO2 antibody

GTX105899_39946_IHC_19011506_305.jpg
GTX105899_WB_19011506_391.jpg
GTX105899_39946_WB_M_Liver_19011506_224.jpg

Cat No. GTX105899

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

293T , A431 , mouse liver

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NQO2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DHQV antibody, DIA6 antibody, NMOR2 antibody, QR2 antibody, NQO2 antibody, ribosyldihydronicotinamide dehydrogenase [quinone] antibody, ribosyldihydronicotinamide dehydrogenase antibody, NAD(P)H menadione oxidoreductase 2, dioxin-inducible antibody, quinone reductase 2 antibody, NRH dehydrogenase [quinone] 2 antibody, NAD(P)H menadione oxidoreductase-1, dioxin-inducible-2 antibody, NRH:quinone oxidoreductase 2 antibody, NAD(P)H dehydrogenase, quinone 2 antibody

Cellular Localization

Cytoplasm

Background

NQO2 (EC 1.10.99.2) is a flavoprotein that catalyzes the 2-electron reduction of various quinones, redox dyes, and the vitamin K menadione. NQO2 predominantly uses dihydronicotinamide riboside (NRH) as the electron donor (summary by Wu et al., 1997 [PubMed 9367528]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX105899_39946_IHC_19011506_305.jpg

GTX105899 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PLC xenograft, using NQO2(GTX105899) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105899_WB_19011506_391.jpg

GTX105899 WB Image

Sample(30 µg whole cell lysate)
A:293T
B:A431(GTX27909)
12% SDS PAGE
GTX105899 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105899_39946_WB_M_Liver_19011506_224.jpg

GTX105899 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse liver
12% SDS PAGE
GTX105899 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169