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NQO2 antibody

Anti-NQO2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105899
Anti-NQO2 antibody used in Western Blot (WB). GTX105899
Anti-NQO2 antibody used in Western Blot (WB). GTX105899

Cat. No. GTX105899

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all NQO2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

Mouse liver , 293T , A431

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NQO2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951018

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

N-ribosyldihydronicotinamide:quinone reductase 2 , DHQV , DIA6 , NMOR2 , QR2

Cellular Localization

Cytoplasm

Background

NQO2 (EC 1.10.99.2) is a flavoprotein that catalyzes the 2-electron reduction of various quinones, redox dyes, and the vitamin K menadione. NQO2 predominantly uses dihydronicotinamide riboside (NRH) as the electron donor (summary by Wu et al., 1997 [PubMed 9367528]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-NQO2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105899

GTX105899 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PLC xenograft, using NQO2(GTX105899) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NQO2 antibody used in Western Blot (WB). GTX105899

GTX105899 WB Image

Sample(30 μg whole cell lysate)
A:293T
B:A431(GTX27909)
12% SDS PAGE
GTX105899 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NQO2 antibody used in Western Blot (WB). GTX105899

GTX105899 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse liver
12% SDS PAGE
GTX105899 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Chen D et al. Biology of Reproduction 2017; (Epub) NQO2 inhibition relieves ROS effects on mouse oocyte meiotic maturation and embryo development.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169