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NR0B1 antibody

Anti-NR0B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105193
Anti-NR0B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105193

Cat No. GTX105193

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NR0B1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

nuclear receptor subfamily 0 group B member 1 , AHC , AHCH , AHX , DAX-1 , DAX1 , DSS , GTD , HHG , NROB1 , SRXY2

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism. [provided by RefSeq]

Database

DATA IMAGES
Anti-NR0B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105193

GTX105193 ICC/IF Image

NR0B1 antibody detects NR0B1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NR0B1 protein stained by NR0B1 antibody (GTX105193) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-NR0B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105193

GTX105193 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NR0B1(GTX105193) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169