APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.4mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human NR0B1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
AHC antibody, AHCH antibody, AHX antibody, DAX-1 antibody, DAX1 antibody, DSS antibody, GTD antibody, HHG antibody, NROB1 antibody, NR0B1 antibody, nuclear receptor DAX1 antibody, nuclear receptor subfamily 0 group B member 1 antibody, nuclear hormone receptor antibody, nuclear receptor DAX-1 antibody, DSS-AHC critical region on the X chromosome protein 1 antibody, nuclear receptor subfamily 0, group B, member 1 antibody
Cellular Localization
Nucleus , Cytoplasm
Background
This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism. [provided by RefSeq]
Database
DATA IMAGES
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GTX105193 ICC/IF Image
NR0B1 antibody detects NR0B1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NR0B1 protein stained by NR0B1 antibody (GTX105193) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.
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GTX105193 IHC-P Image
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NR0B1(GTX105193) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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