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NR1D1 antibody

Orthogonal Overexpression
Anti-NR1D1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113904
Anti-NR1D1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113904
Anti-NR1D1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113904
Anti-NR1D1 antibody used in Western Blot (WB). GTX113904
Anti-NR1D1 antibody used in Western Blot (WB). GTX113904

Cat. No. GTX113904

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all NR1D1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Predict Reactivity

Rat, Sheep, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NR1D1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162728

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear receptor subfamily 1 group D member 1 , EAR1 , REVERBA , REVERBalpha , THRA1 , THRAL , ear-1 , hRev

Cellular Localization

Nucleus

Background

Functions as a constitutive transcriptional repressor. Possible receptor for triiodothyronine.

Database

Research Area

DATA IMAGES

Anti-NR1D1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113904

GTX113904 IHC-P Image

NR1D1 antibody detects NR1D1 protein at nucleus on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
NR1D1 antibody (GTX113904) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NR1D1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113904

GTX113904 IHC-P Image

NR1D1 antibody detects NR1D1 protein at cytosol and nucleus on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
NR1D1 antibody (GTX113904) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NR1D1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113904

GTX113904 ICC/IF Image

NR1D1 antibody detects NR1D1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NR1D1 protein stained by NR1D1 antibody (GTX113904) diluted at 1:400.
Blue: Hoechst 33342 staining.
Anti-NR1D1 antibody used in Western Blot (WB). GTX113904

GTX113904 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NR1D1 antibody (GTX113904) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NR1D1 antibody used in Western Blot (WB). GTX113904

GTX113904 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NR1D1 antibody (GTX113904) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169