GeneTex
United States (US)

NR1D2 antibody [N3C2], Internal

GTX115322_WB_18121410_923.jpg
GTX115322_40282_IFA_18121410_527.jpg

Cat No. GTX115322

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NR1D2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nuclear Receptor Subfamily 1 Group D Member 2,Bd73,Ear-1R,Reverbb,Reverbbeta,Rvr,Nr1D2

Cellular Localization

Nucleus

Background

This gene encodes a member of the nuclear hormone receptor family, specifically the NR1 subfamily of receptors. The encoded protein functions as a transcriptional repressor and may play a role in circadian rhythms and carbohydrate and lipid metabolism. Alternatively spliced transcript variants have been described. [provided by RefSeq]

Database

DATA IMAGES
GTX115322_WB_18121410_923.jpg

GTX115322 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
7.5% SDS PAGE
GTX115322 diluted at 1:1000

GTX115322_40282_IFA_18121410_527.jpg

GTX115322 ICC/IF Image

NR1D2 antibody [N3C2], Internal detects NR1D2 protein at Nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NR1D2 protein stained by NR1D2 antibody [N3C2], Internal (GTX115322) diluted at 1:500.
Blue: Hoechst 33343 staining.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169