GeneTex
United States (US)

NR1H4 antibody

Comparison
GTX117553_40779_20161027_WB_competitor_watermark_18121410_747.jpg
GTX117553_40779_WB_18121410_194.jpg

Cat No. GTX117553

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A549 , H1299 , HCT116 , MCF-7

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NR1H4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nuclear Receptor Subfamily 1 Group H Member 4,Bar,Fxr,Hrr-1,Hrr1,Pfic5,Rip14,Nr1H4

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES
GTX117553_40779_20161027_WB_competitor_watermark_18121410_747.jpg

GTX117553 WB Image

Comparison

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NR1H4 antibody (GTX117553) diluted at 1:500 and competitor's antibody (SC-13063) diluted by 1:100.
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX117553_40779_WB_18121410_194.jpg

GTX117553 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: H1299
C: HCT116
D: MCF-7
10% SDS PAGE
GTX117553 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169