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NR2C2 antibody

Anti-NR2C2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105119
Anti-NR2C2 antibody used in Western Blot (WB). GTX105119
Anti-NR2C2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105119

Cat No. GTX105119

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

H1299 , Molt-4

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NR2C2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

nuclear receptor subfamily 2 group C member 2 , TAK1 , TR4

Cellular Localization

Nucleus

Background

Members of the nuclear hormone receptor family, such as NR2C2, act as ligand-activated transcription factors. The proteins have an N-terminal transactivation domain, a central DNA-binding domain with 2 zinc fingers, and a ligand-binding domain at the C terminus. The activated receptor/ligand complex is translocated to the nucleus where it binds to hormone response elements of target genes (Yoshikawa et al., 1996 [PubMed 8661150]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-NR2C2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105119

GTX105119 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NR2C2(GTX105119) antibody at 1:500 dilution.

Anti-NR2C2 antibody used in Western Blot (WB). GTX105119

GTX105119 WB Image

Sample (30μg whole cell lysate)
A:H1299
B:MOLT4 (GTX27912)
7.5% SDS PAGE
GTX105119 diluted at 1:500

Anti-NR2C2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105119

GTX105119 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using NR2C2(GTX105119) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169