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NR2F6 antibody

Anti-NR2F6 antibody used in IHC (Paraffin sections) (IHC-P). GTX100255
Anti-NR2F6 antibody used in Western Blot (WB). GTX100255
Anti-NR2F6 antibody used in Western Blot (WB). GTX100255

Cat No. GTX100255

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ChIP assay
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

HeLa , Raji , NIH-3T3 , JC

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NR2F6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

nuclear receptor subfamily 2 group F member 6,EAR-2,EAR2,ERBAL2

Cellular Localization

Nucleus

Background

Transcription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type-specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC).

Database

DATA IMAGES
Anti-NR2F6 antibody used in IHC (Paraffin sections) (IHC-P). GTX100255

GTX100255 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using EAR2 (GTX100255) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NR2F6 antibody used in Western Blot (WB). GTX100255

GTX100255 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa
B: Raji
10% SDS PAGE
GTX100255 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NR2F6 antibody used in Western Blot (WB). GTX100255

GTX100255 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
B: JC
10% SDS PAGE
GTX100255 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169