GeneTex
United States (US)

NR5A2 antibody [N2C3]

GTX106024_39974_20160121_WB_19011506_444.jpg
GTX106024_39974_WB_M_liver_19011506_316.jpg
GTX106024_39974_IFA_hESC_19011506_993.jpg

Cat No. GTX106024

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

mouse liver , Aspc1

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Chicken, Pig, Japanese Medaka, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NR5A2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nuclear Receptor Subfamily 5 Group A Member 2,B1F,B1F2,Cpf,Ftf,Ftz-F1,Ftz-F1Beta,Lrh-1,Lrh1,Hb1F-2,Nr5A2

Cellular Localization

Nucleus

Background

Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.

Database

Research Area

DATA IMAGES
GTX106024_39974_20160121_WB_19011506_444.jpg

GTX106024 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NR5A2 antibody [N2C3] (GTX106024) diluted at 1:500.

GTX106024_39974_WB_M_liver_19011506_316.jpg

GTX106024 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX106024 diluted at 1:1000

GTX106024_39974_IFA_hESC_19011506_993.jpg

GTX106024 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using NR5A2(GTX106024) antibody at 1:400 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169