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NR5A2 antibody [N2C3]

Anti-NR5A2 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106024
Anti-NR5A2 antibody [N2C3] used in Western Blot (WB). GTX106024

Cat. No. GTX106024

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NR5A2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

Aspc1

Predict Reactivity

Mouse, Rat, Zebrafish, Sheep, Bovine, Chicken, Pig, Japanese Medaka, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NR5A2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951024

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear receptor subfamily 5 group A member 2 , B1F , B1F2 , CPF , FTF , FTZ-F1 , FTZ-F1beta , LRH-1 , LRH1 , hB1F-2

Cellular Localization

Nucleus

Background

Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.

Database

Research Area

DATA IMAGES

Anti-NR5A2 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106024

GTX106024 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using NR5A2(GTX106024) antibody at 1:400 dilution.

Anti-NR5A2 antibody [N2C3] used in Western Blot (WB). GTX106024

GTX106024 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NR5A2 antibody [N2C3] (GTX106024) diluted at 1:500.

REFERENCE

Application Reference

Suyama A et al. Endocr J 2021; (Epub) Roles of NR5A1 and NR5A2 in the regulation of steroidogenesis by Clock gene and bone morphogenetic proteins by human granulosa cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169