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NRBP1 antibody [5C3]

Anti-NRBP1 antibody [5C3] used in Flow cytometry (FACS). GTX84007
Anti-NRBP1 antibody [5C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84007
Anti-NRBP1 antibody [5C3] used in IHC (Paraffin sections) (IHC-P). GTX84007
Anti-NRBP1 antibody [5C3] used in IHC (Paraffin sections) (IHC-P). GTX84007
Anti-NRBP1 antibody [5C3] used in IHC (Paraffin sections) (IHC-P). GTX84007
Anti-NRBP1 antibody [5C3] used in IHC (Paraffin sections) (IHC-P). GTX84007
Anti-NRBP1 antibody [5C3] used in IHC (Paraffin sections) (IHC-P). GTX84007
Anti-NRBP1 antibody [5C3] used in IHC (Paraffin sections) (IHC-P). GTX84007
Anti-NRBP1 antibody [5C3] used in Western Blot (WB). GTX84007
Anti-NRBP1 antibody [5C3] used in IHC (Paraffin sections) (IHC-P). GTX84007
Anti-NRBP1 antibody [5C3] used in IHC (Paraffin sections) (IHC-P). GTX84007
Anti-NRBP1 antibody [5C3] used in IHC (Paraffin sections) (IHC-P). GTX84007
Anti-NRBP1 antibody [5C3] used in Immunoprecipitation (IP). GTX84007
Anti-NRBP1 antibody [5C3] used in Western Blot (WB). GTX84007

Cat No. GTX84007

Host

Mouse

Clonality

Monoclonal

Clone Name

5C3

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, FACS, IP

Reactivity

Human, Monkey
Package
100 μl ($459)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
IP 2ug/500ul
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human NRBP1 expression vector

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_10730135

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nuclear receptor binding protein 1 , BCON3 , MADM , MUDPNP , NRBP

Background

May play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rho-type GTPases. Binding to the NS3 protein of dengue virus type 2 appears to subvert this activity into the alteration of the intracellular membrane structure associated with flaviviral replication.

Database

DATA IMAGES

Anti-NRBP1 antibody [5C3] used in Flow cytometry (FACS). GTX84007

GTX84007 FACS Image

FACS analysis of HEK293T cells transfected with either NRBP1 plasmid(Red) or empty vector control plasmid(Blue) using GTX84007 NRBP1 antibody [5C3].

Anti-NRBP1 antibody [5C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84007

GTX84007 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with NRBP1 plasmid using GTX84007 NRBP1 antibody [5C3].

Anti-NRBP1 antibody [5C3] used in IHC (Paraffin sections) (IHC-P). GTX84007

GTX84007 IHC-P Image

IHC-P analysis of human breast tissue using GTX84007 NRBP1 antibody [5C3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NRBP1 antibody [5C3] used in IHC (Paraffin sections) (IHC-P). GTX84007

GTX84007 IHC-P Image

IHC-P analysis of human lymphoma tissue using GTX84007 NRBP1 antibody [5C3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NRBP1 antibody [5C3] used in IHC (Paraffin sections) (IHC-P). GTX84007

GTX84007 IHC-P Image

IHC-P analysis of human colon tissue using GTX84007 NRBP1 antibody [5C3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NRBP1 antibody [5C3] used in IHC (Paraffin sections) (IHC-P). GTX84007

GTX84007 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84007 NRBP1 antibody [5C3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NRBP1 antibody [5C3] used in IHC (Paraffin sections) (IHC-P). GTX84007

GTX84007 IHC-P Image

IHC-P analysis of human thyroid carcinoma tissue using GTX84007 NRBP1 antibody [5C3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NRBP1 antibody [5C3] used in IHC (Paraffin sections) (IHC-P). GTX84007

GTX84007 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84007 NRBP1 antibody [5C3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NRBP1 antibody [5C3] used in Western Blot (WB). GTX84007

GTX84007 WB Image

WB analysis of various cell lines using GTX84007 NRBP1 antibody [5C3].
Loading : 35 ug per lane

Anti-NRBP1 antibody [5C3] used in IHC (Paraffin sections) (IHC-P). GTX84007

GTX84007 IHC-P Image

IHC-P analysis of human pancreas carcinoma tissue using GTX84007 NRBP1 antibody [5C3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NRBP1 antibody [5C3] used in IHC (Paraffin sections) (IHC-P). GTX84007

GTX84007 IHC-P Image

IHC-P analysis of human ovary tissue using GTX84007 NRBP1 antibody [5C3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NRBP1 antibody [5C3] used in IHC (Paraffin sections) (IHC-P). GTX84007

GTX84007 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX84007 NRBP1 antibody [5C3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NRBP1 antibody [5C3] used in Immunoprecipitation (IP). GTX84007

GTX84007 IP Image

IP analysis of DDDDK tagged NRBP1 overexpressed HEK293T lysate using GTX84007 NRBP1 antibody [5C3].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

Anti-NRBP1 antibody [5C3] used in Western Blot (WB). GTX84007

GTX84007 WB Image

WB analysis of HEK293T cells transfected with NRBP1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84007 NRBP1 antibody [5C3].
Loading : 5 ug per lane

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459