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NRBP1 antibody [6C11]

Overexpression
Anti-NRBP1 antibody [6C11] used in Flow cytometry (FACS). GTX84003
Anti-NRBP1 antibody [6C11] used in Immunoprecipitation (IP). GTX84003
Anti-NRBP1 antibody [6C11] used in Western Blot (WB). GTX84003
Anti-NRBP1 antibody [6C11] used in Western Blot (WB). GTX84003
Anti-NRBP1 antibody [6C11] used in IHC (Paraffin sections) (IHC-P). GTX84003
Anti-NRBP1 antibody [6C11] used in IHC (Paraffin sections) (IHC-P). GTX84003

Cat No. GTX84003

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6C11

Isotype

 IgG2a

Application

 WB, IHC-P, FACS, IP

Reactivity

 Human, Monkey
Package
100 μl ($459)
See all NRBP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000
IHC-P 1:50
FACS 1:100
IP 2ug/500ul
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human NRBP1 expression vector

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nuclear receptor binding protein 1 , BCON3 , MADM , MUDPNP , NRBP

Background

May play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rho-type GTPases. Binding to the NS3 protein of dengue virus type 2 appears to subvert this activity into the alteration of the intracellular membrane structure associated with flaviviral replication.

Database

DATA IMAGES

Anti-NRBP1 antibody [6C11] used in Flow cytometry (FACS). GTX84003

GTX84003 FACS Image

FACS analysis of HEK293T cells transfected with either NRBP1 plasmid(Red) or empty vector control plasmid(Blue) using GTX84003 NRBP1 antibody [6C11].

Anti-NRBP1 antibody [6C11] used in Immunoprecipitation (IP). GTX84003

GTX84003 IP Image

IP analysis of DDDDK tagged NRBP1 overexpressed HEK293T lysate using GTX84003 NRBP1 antibody [6C11].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

Anti-NRBP1 antibody [6C11] used in Western Blot (WB). GTX84003

GTX84003 WB Image

Overexpression

WB analysis of HEK293T cells transfected with NRBP1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84003 NRBP1 antibody [6C11].
Loading : 5 ug per lane

Anti-NRBP1 antibody [6C11] used in Western Blot (WB). GTX84003

GTX84003 WB Image

WB analysis of various cell lines using GTX84003 NRBP1 antibody [6C11].
Loading : 35 ug per lane

Anti-NRBP1 antibody [6C11] used in IHC (Paraffin sections) (IHC-P). GTX84003

GTX84003 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84003 NRBP1 antibody [6C11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NRBP1 antibody [6C11] used in IHC (Paraffin sections) (IHC-P). GTX84003

GTX84003 IHC-P Image

IHC-P analysis of human breast tissue using GTX84003 NRBP1 antibody [6C11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459