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NRBP1 antibody [7E2]

Overexpression
Anti-NRBP1 antibody [7E2] used in Western Blot (WB). GTX84000
Anti-NRBP1 antibody [7E2] used in Immunoprecipitation (IP). GTX84000
Anti-NRBP1 antibody [7E2] used in Flow cytometry (FACS). GTX84000
Anti-NRBP1 antibody [7E2] used in Western Blot (WB). GTX84000
Anti-NRBP1 antibody [7E2] used in IHC (Paraffin sections) (IHC-P). GTX84000

Cat. No. GTX84000

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7E2

Isotype

 IgG2b

Application

 WB, IHC-P, FACS, IP

Reactivity

 Human, Rat, Dog, Monkey
Package
100 μl ($519)
See all NRBP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:50
FACS 1:100
IP 2ug/500ul
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human NRBP1 expression vector

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear receptor binding protein 1 , BCON3 , MADM , MUDPNP , NRBP

Background

May play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rho-type GTPases. Binding to the NS3 protein of dengue virus type 2 appears to subvert this activity into the alteration of the intracellular membrane structure associated with flaviviral replication.

Database

Research Area

DATA IMAGES

Anti-NRBP1 antibody [7E2] used in Western Blot (WB). GTX84000

GTX84000 WB Image

WB analysis of various cell lines using GTX84000 NRBP1 antibody [7E2].
Loading : 35 ug per lane

Anti-NRBP1 antibody [7E2] used in Immunoprecipitation (IP). GTX84000

GTX84000 IP Image

IP analysis of DDDDK tagged NRBP1 overexpressed HEK293T lysate using GTX84000 NRBP1 antibody [7E2].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

Anti-NRBP1 antibody [7E2] used in Flow cytometry (FACS). GTX84000

GTX84000 FACS Image

FACS analysis of HEK293T cells transfected with either NRBP1 plasmid(Red) or empty vector control plasmid(Blue) using GTX84000 NRBP1 antibody [7E2].

Anti-NRBP1 antibody [7E2] used in Western Blot (WB). GTX84000

GTX84000 WB Image

Overexpression

WB analysis of HEK293T cells transfected with NRBP1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84000 NRBP1 antibody [7E2].
Loading : 5 ug per lane

Anti-NRBP1 antibody [7E2] used in IHC (Paraffin sections) (IHC-P). GTX84000

GTX84000 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84000 NRBP1 antibody [7E2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519