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NRCAM antibody [7D8C5]

Anti-NRCAM antibody [7D8C5] used in Western Blot (WB). GTX60770
Anti-NRCAM antibody [7D8C5] used in ELISA (ELISA). GTX60770
Anti-NRCAM antibody [7D8C5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60770

Cat. No. GTX60770

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7D8C5

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all NRCAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/50
ELISA 1/10000
Not tested in other applications.

Calculated MW

144 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human NRCAM (AA: 1192-1255) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neuronal cell adhesion molecule

Cellular Localization

Cell membrane,Secreted

Background

Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. This gene is also expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-NRCAM antibody [7D8C5] used in Western Blot (WB). GTX60770

GTX60770 WB Image

WB analysis of HEK293 (1) and NRCAM (AA: 1192-1255)-hIgGFc transfected HEK293 (2) cell lysate using GTX60770 NRCAM antibody [7D8C5].

Anti-NRCAM antibody [7D8C5] used in ELISA (ELISA). GTX60770

GTX60770 ELISA Image

ELISA analysis of antigen using GTX60770 NRCAM antibody [7D8C5].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-NRCAM antibody [7D8C5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60770

GTX60770 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60770 NRCAM antibody [7D8C5].
Green : NRCAM
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409