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NRF1 antibody [JM89-63]

Recombinant antibody
Anti-NRF1 antibody [JM89-63] used in Western Blot (WB). GTX00985
Anti-NRF1 antibody [JM89-63] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00985
Anti-NRF1 antibody [JM89-63] used in IHC (Paraffin sections) (IHC-P). GTX00985
Anti-NRF1 antibody [JM89-63] used in Flow cytometry (FACS). GTX00985
Anti-NRF1 antibody [JM89-63] used in IHC (Paraffin sections) (IHC-P). GTX00985
Anti-NRF1 antibody [JM89-63] used in IHC (Paraffin sections) (IHC-P). GTX00985
Anti-NRF1 antibody [JM89-63] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00985
Anti-NRF1 antibody [JM89-63] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00985
Anti-NRF1 antibody [JM89-63] used in IHC (Paraffin sections) (IHC-P). GTX00985

Cat No. GTX00985

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 JM89-63

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μl ($369)
See all NRF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nuclear respiratory factor 1 , ALPHAPAL , NRF1

Cellular Localization

Nucleus

Background

This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014]

Database

Research Area

DATA IMAGES

Anti-NRF1 antibody [JM89-63] used in Western Blot (WB). GTX00985

GTX00985 WB Image

WB analysis of mouse heart tissue lysate using GTX00985 NRF1 antibody [JM89-63].
Dilution : 1:500

Anti-NRF1 antibody [JM89-63] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00985

GTX00985 ICC/IF Image

ICC/IF analysis of PFA-fixed MCF-7 cells using GTX00985 NRF1 antibody [JM89-63].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-NRF1 antibody [JM89-63] used in IHC (Paraffin sections) (IHC-P). GTX00985

GTX00985 IHC-P Image

IHC-P analysis of mouse colon tissue section using GTX00985 NRF1 antibody [JM89-63].

Anti-NRF1 antibody [JM89-63] used in Flow cytometry (FACS). GTX00985

GTX00985 FACS Image

FACS analysis of 293T cells using GTX00985 NRF1 antibody [JM89-63].
Red : primary antibody
Black : unlabelled control
Dilution : 1:100

Anti-NRF1 antibody [JM89-63] used in IHC (Paraffin sections) (IHC-P). GTX00985

GTX00985 IHC-P Image

IHC-P analysis of human thyriod tissue section using GTX00985 NRF1 antibody [JM89-63].

Anti-NRF1 antibody [JM89-63] used in IHC (Paraffin sections) (IHC-P). GTX00985

GTX00985 IHC-P Image

IHC-P analysis of human tonsil tissue section using GTX00985 NRF1 antibody [JM89-63].

Anti-NRF1 antibody [JM89-63] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00985

GTX00985 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX00985 NRF1 antibody [JM89-63].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-NRF1 antibody [JM89-63] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00985

GTX00985 ICC/IF Image

ICC/IF analysis of PFA-fixed SH-SY-5Y cells using GTX00985 NRF1 antibody [JM89-63].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-NRF1 antibody [JM89-63] used in IHC (Paraffin sections) (IHC-P). GTX00985

GTX00985 IHC-P Image

IHC-P analysis of rat brain tissue section using GTX00985 NRF1 antibody [JM89-63].

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369